HSV 1 & 2 Real Time PCR Kit

Herpes simplex virus invades a human body through damaged skin and mucous membranes. During the first phase of pathogenesis, the virus gets into epithelial cells (mucosa of oral cavity, pharynx or genital organs), where its replication occurs. Papules and vesicles, characteristic for herpes infection, appear on mucosa and skin. Genital herpes (GH) is a herpes simplex virus. It remains persistent in men in the urogenital tract, and in women in the cervical canal, vagina, and urethra. Infecting with genital herpes occurs mainly through sexual contact. During viremia in pregnant women, a fetus is infected via the hematogenic route and during childbirth via contact. Herpes infection arising from viremia may result in afflicting several organs at a time. Herpetic esophagitis, pneumonitis, and hepatitis can develop. Damage to the peripheral nervous system can occur in the form of ganglions, ganglioneuroma, reticuloceratids, and polyneuropathy. Central nervous system (CNS) damage with herpes infection most commonly proceeds as encephalitis or meningoencephalitis

ViroGene HSV 1&2 1.0 Real-Time PCR Kit is based on the real-time PCR principle. The pathogen is detected using fluorescent dyes that are incorporated into oligonucleotide probes. The assay utilizes the 5’ exonuclease activity of Taq Polymerase to cleave a dual-labeled fluorescent hydrolysis probe during the extension phase of PCR

 

• Dual Probe Assay
  The highly mutable nature of pathogens often proves challenging in molecular assay design. Traditional qPCR assays are designed so that a single primer and probe set targets one location within the pathogen genome. However, these single-target assays often prove inadequate to detect and quantify viral targets. Sequence polymorphisms in the assay target region can cause impaired primer or probe binding, which may result in significant under-quantification or a false negative result. To eliminate this possibility, the ViroGene HSV 1&2 qPCR assay targets two regions within the pathogen genome, utilizing unique primer and probe sets for each target site. The driving principle behind our multiple-target assay design is that when a polymorphism occurs in one assay target, the additional sets of primers and probes for the alternate target region will still detect and accurately quantify the pathogen and thus reduces the likelihood of under-quantification or a false negative result.

• All kit Components are Liquid Ready to use with enhanced freeze thaw stability (> 30 times)
• Using the highest standards for choice of enzymes on our kits which can tolerates all types of inhibitors found usually in clinical samples with no effect on result quality 

• Common Internal control a wide variety of Assays.    
• Common Reagents and common protocols which ensures running more than one test type on the same run

• Kit components enough to perform 110 Tests, to perform calibration and QC at no Extra cost

 

Item Name	HSV 1 &2
Technology	Real-Time PCR
Type of Analysis	qualitative and quantitative
Target Sequence	gene encoding B glycoprotein (gB)
Specificity	Herpes simplex virus type 1 (HSV-1), 100 % Herpes simplex virus type 2 (HSV-2), 100 %
Sensitivity (LoD)	reaches up to 122.0 copy/ml with the probability of 95 % (on AcroMetrix™ VZV High Plasma Control using manual extraction ViroGene Viral RNA/DNA Extraction Kit)
Linear Range	1010 – 20 copy/ml with precision of ± 0.5 log
Reporting Units	copy/µl
Metrological Traceability	AcroMetrixTM HSV-1 Plasma Panel (AcroMetrix HSV-1) AcroMetrixTM HSV-2 Plasma Panel (AcroMetrix HSV-2)
Extraction/Inhibition Control	Exogenous Internal Control
Validated Specimen *	plasma, serum
Storage	-20 ± 5 °C
Validated Extraction Method	ViroGene Viral RNA/DNA Extraction Kit & MagCore NA Extractor
Validated Instruments *	Applied Biosystems 7300 / 7500 Real-Time PCR System AriaMx Real-Time PCR System CFX Connect™ / CFX96™ / Dx Real-Time PCR Detection System DTlite Real-Time PCR System LightCycler® 2.0 / 480 LineGene 9600 / 9600 Plus QuantStudio™ 3 Real-Time PCR System Rotor-Gene 3000 / 6000 / Q SLAN® Real-Time PCR System
Required Detection Channels	FAM, HEX
Certification	CE IVD