ViroGene PAI-1(4G/5G) QPCR Kit

ViroGene PAI-1(4G/5G) QPCR Kit

Plasminogen activator inhibitor-1(PAI-1), or serpin E1, is a serine protease inhibitor (serpin) encoded by the human SERPINE1 gene. PAI-1 is a major inhibitor of fibrinolysis, a process that prevents blood clots from growing and becoming problematic. Increased PAI-1 activity results in depressed fibrinolytic activity resulting in elevated risk for thrombosis (formation of blood clots)

The normal (wild-type) promoter for the PAI-1 gene contains a tract of five consecutive G residues. However, there is a mutant form of the PAI-1 promoter that has been identified that only contains four consecutive G residues. This mutant promoter causes higher amounts of PAI-1 to be made compared to the wild-type. Increased amounts of PAI-1 inhibit more tPA and uPA and, therefore, inhibits fibrinolysis more than the wild-type gene, resulting in an elevated risk of thrombosis.

Homozygous wild-type (5G/5G) – Normal PAI-1 activity and normal risk of thrombosis. Heterozygous (4G/5G) – Increased PAI-1 activity resulting in depressed fibrinolysis and increased risk of thrombosis. Homozygous mutant (4G/4G) – Significantly increased PAI-1 activity resulting in depressed fibrinolysis and increased risk of thrombosis

This genotype result is one-factor affecting thrombosis risk, and other genetic and clinical factors should also be considered. Multiple thrombosis risk factors are usually additive. Additional risk factors for the development of thrombosis include: Older age, surgery, obesity, prolonged travel, immobility, hospitalization, oral contraceptive use, hormonal replacement therapy, pregnancy, and malignancy

Indication in vitro diagnostic medical device
Regulatory Status CE IVD
Intended User For professional use in laboratories with trained staff
Technology Real-time PCR
Type of Analysis Qualitative
Target Sequence Polymorphism in promoter of the PAI-1 gene in site 675
Analytical Sensitivity (LoD) Reaches up to 2 ng/μl
Diagnostic Specificity 100 %
Diagnostic Sensitivity 100 %
Validated Specimen Whole blood
Storage -20 ± 5 °C
Validated Extraction Methods ViroGene DNA Extraction Kit & NA16 MagCore NA Extractor
Instruments Applied Biosystems 7300 / 7500 Real-Time PCR System
AriaMx Real-Time PCR System
LightCycler® 2.0 / 480
CFX Connect™ / CFX96™ / Dx Real-Time PCR Detection System
LineGene 9600 Plus
Mic qPCR Cycler
QuantStudio™ 3 / 5 Real-Time PCR System
Rotor-Gene 3000/6000/Q*
SLAN® Real-Time PCR System
StepOne™ / StepOnePlus™ Real-Time PCR System
Required Detection Channels FAM, HEX